rat anti ollas antibody  (Novus Biologicals)

Journal: bioRxiv

Article Title: The conserved ATPase PCH-2 controls the number and distribution of crossovers by antagonizing crossover formation in C. elegans

doi: 10.1101/2024.08.13.607819

Figure Lengend Snippet: A. Representative images of nuclei in different stages of meiotic prophase in control animals and pch-2 mutants stained for DAPI (magenta) and GFP::MSH-5 (green) Scale bar is 5 um. B. Scatter plot showing average GFP::MSH-5 foci per row of germline nuclei in control animals (yellow) and pch-2 mutants (blue) from the transition zone to late pachytene, normalized to 100. The line represents a rolling average of four rows. C. Representative images of nuclei in different stages of meiotic prophase in him-3 R93Y mutants (left) and pch-2 ; him-3 R93Y double mutants (right), stained for DAPI (magenta) and GFP::MSH-5 (green). D. Scatter plot showing average GFP::MSH-5 foci per row in him-3 R93Y (brown) and pch-2;him-3 R93Y mutants (pink) from the transition zone to late pachytene, normalized to 100. The line represents a rolling average of four rows. Similar data is provided for a control germline (opaque yellow) for comparison. E. Representative images of meiotic nuclei in control animals and pch-2 mutants stained for DAPI (blue), GFP::MSH-5 (green), and OLLAS::COSA-1 (red). Yellow circles indicate GFP::MSH-5 without OLLAS::COSA-1. Scale bar is 4um. F. Scatter plot showing average GFP::MSH-5 (green) and OLLAS::COSA-1 (red) foci per row in the last five rows of the germline in control animals and pch-2 mutants. The line represents a rolling average of 2 rows. G. Swarm plot showing number of GFP::MSH-5 foci in control (yellow) and pch-2 mutant (blue) nuclei with less than 6 OLLAS::COSA-1 foci (left) and 6 OLLAS::COSA-1 foci (right). Error bars represent SEM. A * indicates a p-value less than 0.05 and a ** indicates a p-value < 0.01.

Article Snippet: The following primary antibodies were used at the indicated dilutions: alpaca anti-GFP Booster (ChromoTek, gb2AF488) was used at 1:1000; rat anti-OLLAS (Invitrogen, PIMA516125) was used at 1:1000; and rabbit anti PCH-2 (Bhalla 2014) was used at 1:500.

Techniques: Control, Staining, Comparison, Mutagenesis

Journal: bioRxiv

Article Title: The conserved ATPase PCH-2 controls the number and distribution of crossovers by antagonizing crossover formation in C. elegans

doi: 10.1101/2024.08.13.607819

Figure Lengend Snippet: Gray scale images of control and pch-2 mutant nuclei stained for DAPI, GFP::MSH-5 and OLLAS::COSA-1. Scale bar in image is 4 um.

Article Snippet: The following primary antibodies were used at the indicated dilutions: alpaca anti-GFP Booster (ChromoTek, gb2AF488) was used at 1:1000; rat anti-OLLAS (Invitrogen, PIMA516125) was used at 1:1000; and rabbit anti PCH-2 (Bhalla 2014) was used at 1:500.

Techniques: Control, Mutagenesis, Staining